Disruption/Why are my tissue sections damaged?

    Disruption: microscopic damage to the tissue within the section


    Causes

    Remedies

    Rough grossing

    • Change trimming and/or scalpel blades more frequently to prevent damage

    Poor tissue processing

    • Soak block on ice bath to soften overprocessed, hard tissue; reprocess appropriately if under processed

    Rough handling in water bath to dislodge wrinkles

    • Approach sections more gently with forceps or needles

    Floating in water bath too long or in water that is too hot

    • Pick up sections in a timelier manner or reduce temperature in water bath